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Diploma Courses – Duration: 20-25Days
Diploma in PCR Techniques
- Extract ion of DNA
- Restriction Digestion
- Southern Blotting
- Introduction to PCR
- General PCR
- Study of other types of PCR
- Analysis by spectrophotometric method
Diploma in Enzyme Technology
- Fundamentals of Enzymes
- Standard curve of PNP (Purine Bucleoside Phosphorylase )
- Enzyme extraction and purification by salt precipitation
- To determine Enzyme Activity and to check the protein linearity.
- Enzyme kinetics studies for various enzymes – pH
- Enzyme kinetics studies for various enzymes – Temperature
- Enzyme kinetics studies for various enzymes – Substrate concentration
- Immobilization of enzymes
- Analysis of Enzymes activity against time
- Industrial Application
Diploma Recombinant Technology
- Isolation of DNA from Bacteria
- Isolation of Proteins from Bacteria
- Rapid isolation of Plasmid DNA
- Quantitative analysis of DNA by Colorimetric method (DPA Method)
- Quantitative analysis of DNA by Spectrophotometric method
- Restriction digestion of genomic DNA
- Restriction digestion of Plasmid DNA
- Agarose Gel Electrophoresis and PAGE
- Southern Hybridization
- Western blotting
- Electroelution of DNA from Agarose gel
- DNA ligation
- GFP gene cloning
- Bacterial transformation
- Gene expression
- Analysis of Observations
- Industrial Applications
Diploma Clinical Microbiology
- Fundamentals of Microbiology
- Sterilization techniques
- Microbial culture media preparation and its use
- Microbial culturing techniques
- Isolation of Pure culture
- Identification of micro-organisms by various staining techniques
- Identification of micro-organisms by various biochemical tests
- Detection of micro-organisms from clinical samples – pus
- Detection of micro-organisms from clinical samples – stool
- Detection of micro-organisms from clinical samples – urine
- Detection of micro-organisms from clinical samples – sputum
- Detection of micro-organisms from clinical samples – blood
- Detection of micro-organisms from clinical samples – saliva
- Antibiotic assay (MIC)
- Antibiotic sensitivity
- Industrial Applications
Diploma Industrial Microbiology
- Fundamentals of Microbiology and industrially important micro-organisms, their products
- Sterilization techniques
- Microbial culture media preparation and its use (Different types of growth Media depending upon the requirement of different types of organism)
- Media Pouring & Microbial culturing techniques
- Isolation of Pure culture from soil, water, air etc.
- Identification of micro-organisms by various staining techniques
- Identification of micro-organisms by various biochemical tests
- Microbial growth curve preparation
- Fundamentals of Bioreactor / Fermenter
- Alcohol fermentation using yeast
- Production of Citric acid from fungi
- Production of antibiotic from fungi
- Production of Single cell protein
- Methylene blue reduction test for milk
- Industrial Applications from milk
Diploma Bio-fertilizer & Biopesticides
- Fundamentals of Biofertilizer technology
- Fundamentals of Biopesticide technology
- Sterilization techniques
- Microbial culture media preparation
- Isolation of micro-organism
- Identification of micro-organism
- Pure culture preparation
- Mass production of micro-organism
- Incubation and Harvesting the micro-organism
- Formulation of the product
- Quality analysis
- Degradation of pesticide in soil and estimation of its residue.
- Determination of LD 50 for common pesticide / weedicides
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